To view a list of Dr. Bundle's research publications, click here.
research projects in the group are focused on the challenging task
of reproducibly creating nanomolar oligosaccharide based inhibitors
for carbohydrate binding proteins, and the elucidation of the structural
basis of unique molecular recognition of carbohydrate antigens by
the immune system. Chemical synthesis and structural biology are
central elements in nearly all projects.
To date successful outcomes have included a nanomolar inhibitor of the Shiga
like toxin from E. coli O157:H7 (Nature, 403,
669-672, 2000) followed by a second generation approach that has resulted in
a simplified molecule that can neutralize this toxin in-vivo (Proc.
Natl. Acad. Sci (USA), 105,
Research funding in the form of operating and equipment grants to support these general objectives is derived from several sources, Alberta Ingenuity and the Natural Science and Engineering Research Council (NSERC), Graduate students and postdoctoral fellows also hold competitive fellowships with some of these granting agencies.
Currently funded research projects include: Multivalence assisted supramolecular assembly in biological sysytems
E. coli Shiga-like toxins Stx-1 and Stx-2
Brucella abortus, Brucella melitensis and Brucella suis
Group facilities and special equipment
The group is well equipped to carry out synthetic and biological aspects of research on complex carbohydrates. In addition to the standard items for performing organic chemistry, other equipment includes automated peptide synthesizer, preparative HPLC for organic and protein samples, titration microcalorimeter, UV and visible ELISA plate readers and washers, centrifuges, ultrafiltration devices and spectrophotometers. We maintain a dedicated tissue culture facility with the capability to grow mammalian cells in stationary flasks, roller bottles, shaker flasks and bioreactors. In addition to a full complement of PC to support routine work in the group, we utilizeDepartmental computer clusters and supercomputing facilities for molecular modeling.
services within the Department are amongst the best in North America
and include electronic, glass blowing and machine shops (www.chem.ualberta.ca/facres.htm).
Resources available include state-of-the-art facilities in:
- Nuclear magnetic resonance (NMR) spectroscopy, Varian700, 600,
500, 400 and 300 MHz spectrometers ( http://nmr.chem.ualberta.ca/AOWWW/index.htm)
- Mass Spectrometry,
- X-ray crystallography,
- Surface plasmon resonance.
Also available on campus at the University of Alberta are the facilities
and resources of the following:
- 800 MHz National High Field Nuclear Magnetic Resonance Centre
- Institute for Biodesign,
- Health Sciences Laboratory Animal Services
- Alberta Research Council’s Carbohydrates and Biotechnology
- The Protein Engineering Center of Excellence (PENCE).
- The Lemieux Collection of Complex Carbohydrate Compounds,
- The Chembiomed Collection of Compound Antibodies and Antibody