If you are not sure which FP is right for your application, you should explore my Introduction to fluorescent proteins page or the fantastic resources available at Molecular Expressions. All FPs listed here are described in our Publications.
GECO series of calcium ion indicators
Enter the name for this tabbed section: B-GECO
B-GECO (ACCESSION JN258412) is available through Addgene
- Mammalian expression plasmid with CMV promoter
Enter the name for this tabbed section: G-GECO
G-GECO1 (ACCESSION JN258413), G-GECO1.1 (ACCESSION JN258414), and G-GECO1.2 (ACCESSION JN258415) are available through Addgene.
- Mammalian expression plasmid with CMV promoter
- Bacterial expression plasmid with arabinose-inducible promoter (pTorPE)
Enter the name for this tabbed section: R-GECO
R-GECO1 (ACCESSION JN258411) is available through Addgene
- Mammalian expression plasmid with CMV promoter
- Mammalian expression plasmid with NLS
- Bacterial expression plasmid with arabinose-inducible promoter (pTorPE)
Enter the name for this tabbed section: GEM-GECO
GEM-GECO1 (ACCESSION JN258409) is available through Addgene
- Mammalian expression plasmid with CMV promoter
- Mitochondrial targeting (CMV-mito-GEM-GECO1)
- Bacterial expression plasmid with arabinose-inducible promoter (pTorPE)
Enter the name for this tabbed section: GEX-GECO
GEX-GECO1 (ACCESSION JN258410) is available through Addgene
- Mammalian expression plasmid with CMV promoter
- Bacterial expression plasmid with arabinose-inducible promoter (pTorPE)
Dimerization dependent fluorescent proteins (ddFPs)
Enter the name for this tabbed section: ddRFP
Enter the name for this tabbed section: ddGFP
Enter the name for this tabbed section: ddYFP
Photoconvertible fluorescent proteins
Enter the name for this tabbed section: mClavGR2
mClavGR2: Distributed by Allele Biotechnology
Enter the name for this tabbed section: mMaple
mMaple: Distributed by Allele Biotechnology
Blue fluorescent proteins
Enter the name for this tabbed section: EBFP2
Enter the name for this tabbed section: mKalama
Enter the name for this tabbed section: mBlueberry2
mBlueberry2 (GenBank EF517319): You will need to request directly from REC. The University of Alberta is a signatory of the UBMTA. If your institution is also a signatory, please fill out, sign, and return this form to REC. Regular mail is fine. If your institution is not a signatory, use this form.
Teal fluorescent proteins
Enter the name for this tabbed section: mTFP1
Enter the name for this tabbed section: mTFP0_7
Green fluorescent proteins
Enter the name for this tabbed section: mWasabi
Yellow fluorescent proteins
Enter the name for this tabbed section: mAmetrine
Enter the name for this tabbed section: mAmetrine1_1
mAmetrine1.1 (~1.5x better photostability): Available through Addgene
Red fluorescent proteins
Enter the name for this tabbed section: cpmCherry
Enter the name for this tabbed section: mNectarine
mNectarine (Accession FJ439505): Available from Addgene. Please note that this protein has relatively poor photostability.
Enter the name for this tabbed section: mFruits
mFruit Variants: The Campbell lab does not distribute the cDNA for any of the mFruit variants as described by Shaner et al. Nature Biotech. (2004). Most of these variants are now available from Clontech.
Enter the name for this tabbed section: tdTomato
FRET constructs
For constructs not listed here, you will need to request directly from REC. The University of Alberta is a signatory of the UBMTA. If your institution is also a signatory, please fill out, sign, and return this form to REC. Regular mail is fine. If your institution is not a signatory, use this form.
Excel file with spectra for mAmetrine1, mBlueberry2, EBFP2, mKalama1, mTFP1, mWasabi available here. The Tsien lab has posted an Excel file with the spectra of many common fluorescent proteins.