Amr El-Hawiet, Yajie Chen, Km Shams-Ud-Doha, Elena N Kitova, Yves St-Pierre, and John S. Klassen "High Throughput, Label- and Immobilization-Free Screening of Human Milk Oligosaccharides Against Lectins" Analytical chemistry2017 Accepted!

Ling Han, Luis Morales, Michele R Richards, Elena N Kitova, Simonetta Sipione, and John S. Klassen "Investigating the Influence of Membrane Composition on Protein-Glycolipid Binding Using Nanodiscs and Proxy Ligand ESI-MS" Analytical chemistry2017 Accepted!

Jun Li, Michele R. Richards, Elena N. Kitova, and John S. Klassen "Delivering Transmembrane Peptide Complexes to the Gas Phase Using Nanodiscs and Electrospray Ionization" J Am Soc Mass Spectrom.2017 Accepted!

Km Shams-Ud-Doha, Elena N. Kitova, Pavel I. Kitov, Yves St-Pierre, and John S. Klassen "Human Milk Oligosaccharide Specificities of Human Galectins. Comparison of Electrospray Ionization Mass Spectrometry and Glycan Microarray Screening Results" Analytical chemistry 2017, 89(9):4914-4921

Wei Zhang, Alexander W Lohman, Yevgeniya Zhuravlova, Xiaocen Lu, Matthew D Wiens, Hiofan Hoi, Sine Yaganoglu, Manuel A Mohr, Elena N Kitova, John S Klassen, Periklis Pantazis, Roger J Thompson& Robert E Campbell "Optogenetic control with a photocleavable protein, PhoCl" Nature Methods 2017, 14:391-394

Ling Han, Elena N. Kitova and John S. Klassen"Detecting Protein-Glycolipid Interactions using Glycomicelles and CaR-ESI-MS" J Am Soc Mass Spectrom. 2016, 27:1878

Jun Li, Michele R. Richards, Dhanashri Bagal, Iain D. G. Campuzano, Elena N. Kitova, Zi Jian Xiong, Gilbert G. Privé, and John S. Klassen "Characterizing the Size and Composition of Saposin A Lipoprotein Picodiscs" Analytical chemistry 2016, 88 (19): 9524-9531 Open Access Link

Ling Han (contributed equally), Km Shams-Ud-Doha (contributed equally), Elena N Kitova, John S Klassen"Screening Oligosaccharide Libraries against Lectins Using the Proxy Protein ESI-MS assay" Analytical chemistry 2016, 88(16):8224-8231

Lan Liu, Iwona Siuda, Michele Richards, Justin Renaud, Elena Kitova, Paul Mayer, Peter Tieleman, Todd Lowary andJohn S. Klassen"Structure and Stability of Carbohydrate-Lipid Interactions. Methylmannose Polysaccharide-Fatty Acid Complexes" ChemBioChem. 2016, 17(16):1571-1578

Jun Li, Xuxin Fan, Elena N Kitova, Chunxia Zou, Christopher W. Cario, Luiz Eugenio, Kenneth KS Ng, Zi Jiang Xiong, Gilber G. Prive and John S. Klassen "Screening Glycolipids Against Proteins in vitro using Picodiscs and Catch-and-Release Electrospray Ionization Mass Spectrometry" Analytical chemistry 2016, 88(9):4742-4750

Reza R. Darestani, Philip Winter, Elena N. Kitova, Jack A. Tuszynski and John S. Klassen " Screening Anti-Cancer Drugs against Tubulin using Catch-and-Release Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom. 2016, 27(5):876-885

Yuyu Yao, Michele R. Richards, Elena N. Kitova and John S. Klassen " Influence of Sulfolane on ESI-MS Measurements of Protein–Ligand Affinities. J Am Soc Mass Spectrom. 2016, 27(3) : 498-506

Jingjing Zhang, Elena N. Kitova, Jun Li, Luiz Eugenio, Kenneth KS Ng and John S. Klassen " Localizing Carbohydrate Binding Sites in Proteins Using Hydrogen/Deuterium Exchange Mass Spectrometry" J Am Soc Mass Spectrom. 2016, 27(1):83-90

Amr El-Hawiet, Elena N. Kitova, and John S. Klassen "Recognition of human milk oligosaccharides by bacterial exotoxins" Glycobiology. 2015, 25(8):845-54

Simon Ng, Edith Lin, Pavel I. Kitov, Katrina F. Tjhung, Oksana O. Gerlits, Lu Deng, BrianKasper, Amika Sood, Beth M. Paschal, Ping Zhang, Chang-Chun Ling, John S. Klassen,Christopher J. Noren, Lara K. Mahal, Robert J. Woods, Leighton Coates, and Ratmir Derda "Genetically-encoded Fragment-based Discovery ofGlycopeptide Ligands for Carbohydrate-binding Proteins" J. Am. Chem. Soc., 2015, 137, 5248-5251

Ling Han, Elena N. Kitova, Jun Li, Sanaz Nikjah, Hong Lin, Benjamin Pluvinage, Alisdair B. Boraston, and John S. Klassen "Protein-Glycolipid Interactions Studied in vitro using ESI-MS and Nanodiscs. Insights into the Mechanisms and Energetics of Binding" Anal Chem. 2015, 87, 4888-4896

Aneika C. Leney, Reza Rezaei Darestani, Jun Li, Sanaz Nikjah, Elena N. Kitova, Chunxia Zou, Christopher W. Cairo, Zi Jian Xiong, Gilbert G. Privé , and John S. Klassen "Picodiscs for Facile Protein-Glycolipid Interaction Analysis" Anal Chem. 2015, 87, 4402-4408

Carlos A. Sanhueza, Jonathan Cartmell, Amr El-Hawiet, Adam Szpacenko, Elena N. Kitova, Rambod Daneshfar, John Klassen, Kenneth K.-S. Ng, Luiz Eugenio, Pavel I. Kitov, and David R. Bundle "Evaluation of a Focused Virtual Library of Heterobifunctional Ligands for Clostridium difficile Toxins" Organic & Biomolecular Chemistry. 2015, 13(1), 283-298

Ling Han, Elena N. Kitova, Ming Tan, Xi Jiang, Benjamin Pluvinage, Alisdair B. Boraston and John S. Klassen "Affinities of Human Histo-Blood Group Antigens for Norovirus Capsid Protein Complexes" Glycobiology. 2015, 25(2), 170-180

Yuyu Yao, Km Shams-Ud-Doha, Rambod Daneshfar, Elena N. Kitova and John S. Klassen "Quantifying Protein-Carbohydrate Interactions Using Liquid Sample Desorption Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom. 2015, 26(1), 98-106

Perrin Baker, Tyler Ricer, Patrick J. Moynihan, Elena N. Kitova, Marthe T. C. Walvoort, Dustin J. Little, John C. Whitney, Karen Dawson, Joel T. Weadge, Howard Robinson, Dennis E. Ohman, Jeroen D. C. Codée, John S. Klassen, Anthony J. Clarke, P. Lynne Howell "P. aeruginosa SGNH Hydrolase-Like Proteins AlgJ and AlgX Have Similar Topology but Separate and Distinct Roles in Alginate Acetylation" PLoS Pathog. 2014, 10(8), e1004334

Elena N. Kitova, Amr El-Hawiet, and John S. Klassen "Screening Carbohydrate Libraries for Protein Interactions using the Direct ESI-MS Assay. Applications to Libraries of Unknown Concentration" Journal of The American Society for Mass Spectrometry. 2014, 25(11), 1908-1916

Ling Han, Ming Tan, Ming Xia, Elena N. Kitova, Xi Jiang, and John S. Klassen "Gangliosides are Ligands for Human Noroviruses" J. Am. Chem. Soc., 2014, 136(36), 12631-12637

Aneika C Leney, Xuxin Fan, Elena N Kitova, John S. Klassen "Nanodiscs and Electrospray Ionization Mass Spectrometry. A Novel Tool for Screening Glycolipids Against Proteins" Anal. Chem., 2014, 86(11), 5271-5277

Lan Liu, Nobar Jalili, Alyson Baergen, Simon Ng, Justin Bailey, Ratmir Derda, John S. Klassen "Fluorine Bonding Enhances the Energetics of Protein-Lipid Binding in the Gas Phase" Journal of The American Society for Mass Spectrometry. 2014, 25(5), 751-757

Lan Liu, Alyson Baergen, Klaus Michelsen, Elena N. Kitova, Paul D. Schnier, John S. Klassen "Energetics of Intermolecular Hydrogen Bonds in a Hydrophobic Protein Cavity" Journal of The American Society for Mass Spectrometry. 2014, 25(5), 742-750

Wolfram F, Kitova EN, Robinson H, Walvoort M, Codée JD, Klassen JS, Howell PL "Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG" J Biol Chem. 2014, 289(9),6006-6019

Murase T, Eugenio L, Schorr M, Hussack G, Tanha J, Kitova EN, Klassen JS, Ng KK "Structural Basis for Antibody Recognition in the Receptor-Binding Domains of Toxins A and B from Clostridium difficile" J Biol Chem. 2014, 289(4), 2331-2343

Jafari MR, Deng L, Kitov PI, Ng S, Matochko WL, Tjhung KF, Zeberoff A, Elias A, Klassen JS, Derda R "Discovery of Light-Responsive Ligands through Screening of a Light-Responsive Genetically Encoded Library" ACS Chem Biol. 2014, 9(2), 443-450

Hong Lin, Elena N. Kitova, John S. Klassen "Measuring Positive Cooperativity Using the Direct ESI-MS Assay. Cholera Toxin B Subunit Homopentamer Binding to GM1 Pentasaccharide" Journal of The American Society for Mass Spectrometry. 2014, 25(1), 104-110

Ling Han, Elena N. Kitova, Ming Tan, Xi Jiang, John S. Klassen "Identifying Carbohydrate Ligands of a Norovirus P Particle using a Catch and Release Electrospray Ionization Mass Spectrometry Assay" Journal of The American Society for Mass Spectrometry. 2014, 25(1), 111-119

Hong Lin, Elena N. Kitova , and John S. Klassen "Quantifying Protein–Ligand Interactions by Direct Electrospray Ionization-MS Analysis: Evidence of Nonuniform Response Factors Induced by High Molecular Weight Molecules and Complexes" Anal. Chem., 2013, 85(19), 8919-8922

Amr El-Hawiet , Elena N. Kitova , and John S. Klassen "Quantifying Protein Interactions with Isomeric Carbohydrate Ligands Using a Catch and Release Electrospray Ionization-Mass Spectrometry Assay" Anal. Chem., 2013, 85(16), 7637-7644

Yixuan Zhang, Lu Deng, Elena N. Kitova, John S. Klassen "Dissociation of Multisubunit Protein–Ligand Complexes in the Gas Phase. Evidence for Ligand Migration" Journal of The American Society for Mass Spectrometry. 2013, 24, 1573-1583

Lu Deng, Elena N. Kitova, John S. Klassen. "Mapping Protein–Ligand Interactions in the Gas Phase Using a Functional Group Replacement Strategy. Comparison of CID and BIRD Activation Methods" Journal of The American Society for Mass Spectrometry. 2013, 24, 988-996

Fumie X. Sunahori, Guochun Yang, Elena N. Kitova, John S. Klassen and Yunjie Xu. "Chirality recognition of the protonated serine dimer and octamer by infrared multiphoton dissociation spectroscopy" Phys. Chem. Chem. Phys., 2013, 15, 1873-1886

Lu Deng, Elena N. Kitova, and John S. Klassen. "Dissociation Kinetics of the Streptavidin-Biotin Interaction Measured using Direct Electrospray Ionization Mass Spectrometry Analysis" Journal of The American Society for Mass Spectrometry. 2013, 24(1), 49-56

Ling Han, Pavel I. Kitov, Elena N. Kitova, Ming Tan, Leyi Wang, Ming Xia, Xi Jiang, and John S. Klassen. "Affinities of Recombinant Norovirus P Dimers for Human Blood Group Antigens" Glycobiology. 2013, 23(3), 276-285

Deng, Lu; Broom, Aron; Kitova, Elena; Richards, Michele; Zheng, Ruixiang; Shoemaker, Glen; Meiering, Elizabeth; Klassen, John "Kinetic Stability of the Streptavidin-Biotin Interaction Enhanced in the Gas Phase " J. Am. Chem. Soc., 2012, 134(40), 16586-16596

Yixuan Zhang, Lan Liu, Rambod Daneshfar, Elena N. Kitova, Caishun Li, Feng Jia, Christopher W. Cairo and John S. Klassen "Protein-Glycosphingolipid Interactions Revealed using Catch-and-Release Mass Spectrometry" Anal. Chem., 2012, 84(18), 7618-7621

Elena N. Kitova, Igor Sinelnikov, Lu Deng and John S. Klassen "Thermal Dissociation of Streptavidin Homotetramer in the Gas Phase. Subunit Loss versus Backbone Fragmentation" Int. J. Mass spectrom. , 2013, 345-347, 97-103.

Hong Lin, Elena N. Kitova, Margaret A. Johnson, Luiz Eugenio, Kenneth K.S. Ng, and John S. Klassen "Electrospray Ionization-Induced Protein Unfolding" J. Am. Soc. Mass Spectrom, 2012, 23(12), 2122-2131

Lan Liu, Yu Bai, Nian Sun, Li Xia, Todd L. Lowary and John S. Klassen "Carbohydrate–Lipid Interactions. Affinities of Methylmannose Polysaccharides for Lipids in Aqueous Solution" Chemistry - A European Journal, 2012, 18(38), 12059-12067

El-Hawiet, A., Kitova, E.N.,and Klassen, J.S. "Quantifying Carbohydrate-Protein Interactions by Electrospray Ionization Mass Spectrometry Analysis" Biochemistry, 2012, 51(21), 4244-4253

El-Hawiet, A., Kitova, E.N., Arutyunov, D., Szymanski, C.M., and Klassen, J.S. "Quantifying ligand binding to large protein complexes using electrospray ionization mass spectrometry" Anal.Chem., 2012, 84(9), 3867–3870

Lan Liu, Klaus Michelsen, Elena N. Kitova, Paul D. Schnier, Alex Brown, and John S. Klassen "Deuterium Kinetic Isotope Effects on the Dissociation of a Protein–Fatty Acid Complex in the Gas Phase" J. Am. Chem. Soc. 2012, 134, 5931–5937

Liu, Lan; Michelsen, Klaus; Kitova, Elena; Schnier, Paul; Klassen, John "Energetics of Lipid Binding in a Hydrophobic Protein Cavity" J. Am. Chem. Soc. 2012, 134, 3054-3060

Elena N. Kitova, Amr El-Hawiet, Paul D. Schnier and John S. Klassen "Reliable Determinations of Protein-Ligand Interactions by Direct ESI-MS Measurements. Are We There Yet?" J. Am. Soc. Mass Spectrom 2012, 23, 431-441

Mahendra S. Sandbhor, Naoto Soya, Amgad Albohy, R. Blake Zheng, Jonathan Cartmell, David R. Bundle, John S. Klassen, and Christopher W. Cairo "Substrate Recognition of the Membrane-Associated Sialidase NEU3 Requires a Hydrophobic Aglycone" Biochemistry 2011, 50, 6753-6762

Ping Zhang, Nahid Razi, Luiz Eugenio, Messele Fentabil, Elena N. Kitova, John S. Klassen, David R. Bundle, Kenneth K.-S. Ng and Chang-Chun Ling "Ligands for C. difficile toxins:Unexpected structure of a pentasaccharide produced by enzymatic fucosylation and corrected annotation in a popular screening library" Chem. Comm. 2011, 7, 12397-12399

Isabelle Hug, Blake Zheng, Bela Reiz, Randy Whittal, Messele A. Fentabil, John S. Klassen, and Mario F. Feldman "Exploiting bacterial glycosylation machineries for the synthesis of a
Lewis antigen-containing glycoprotein " J. Biol. Chem. 2011, 284, 37887-37894

Messele A. Fentabil, Rambod Daneshfar, Elena N. Kitova and John S. Klassen "Blackbody Infrared Radiative Dissociation of Protonated Oligosaccharides" J. Am. Soc. Mass Spectrom, 2011, 22, 2171-2178

Kitova EN, Soya N, Klassen JS. "Identifying Specific Small Molecule Interactions Using
Electrospray Ionization Mass Spectrometry" Anal. Chem. 2011, 83, 5160–5167

Amr El-Hawiet, Elena N. Kitova, Pavel Kitov, Luiz Eugenio, Kenneth K.S. Ng, George L. Mulvey, Tanis C. Dingle, Adam Szpacenko, Glen D. Armstrong and John S. Klassen "Binding of Clostridium difficile Toxins to Human Milk Oligosaccharides" Glycobiology, 2011, 21,1217-1227 

Liu L, Michelsen K, Kitova EN, Schnier PD, Klassen JS. "Evidence that Water Can Reduce the Kinetic Stability of Protein-Hydrophobic Ligand Interaction" J. Am. Chem. Soc. , 2010, 132, 17658-17660

Soya N, Fang Y, Palcic MM, Klassen JS. "Trapping and Characterization of Covalent Intermediates of Mutant Retaining Glycosyltransferases" Glycobiology , 2011, 21, 547-552

Liu L, Kitova EN, Klassen JS. "Quantifying Protein-Fatty Acid Interactions using Electrospray Ionization Mass Spectrometry " J Am Soc Mass Spectrom, 2011, 22, 310-318

Hakansson K and Klassen JS. "Ion Activation Methods for Tandem Mass Spectrometry"Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities, and Biological Applications, Second Edition 2010, Chapter 16 (Editor: Richard B. Cole), WILEY, 571-630.

El-Hawiet A, Kitova EN, Liu L, Klassen JS. "Quantifying Labile Protein-Ligand Interactions using Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom 2010, 21, 1893-1899

Deng L, Sun N, Kitova EN, Klassen JS. "Direct Quantification of Protein-Metal Ion Affinities by Electrospray Ionization Mass Spectrometry" Anal. Chem. 2010, 82, 2170-2174

Sun N, Soya N, Kitova EN, Klassen JS. "Nonspecific Interactions between Proteins and Charged Biomolecules in Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom 2010, 21, 472-481

Liu L, Bagal D, Kitova EN, Schnier PD, Klassen JS. "Hydrophobic Protein - Ligand Interactions Preserved in the Gas Phase" J. Am. Chem. Soc 2009, 131, 15980-15981

Soya N, Shoemaker GK, Palcic MM, Klassen JS. "Comparative Study of Substrate and Product Binding to the Human ABO(H) Blood Group Glycosyltransferases" Glycobiology 2009, 19, 1224-1234

Bagal D, Kitova EN, Liu L, El-Hawiet A, Schnier PD, Klassen JS. "Gas Phase Stabilization of Noncovalent Protein Complexes Formed by Electrospray Ionization" Anal. Chem. 2009, 81, 7801-7806.

Kitova EN, Mulvey GL, Dingle T, Sinelnikov I, Wee S, Griener TP, Armstrong GD, Klassen JS. "Assembly and stability of the shiga toxins investigated by electrospray ionization mass spectrometry" Biochemistry 2009, 48, 5365-5374.

Humphries RM, Donnenbberg MS, Strecher J, Kitova EN, Klassen JS, Cui L, Griener TP, Mulvey GL, Armstrong GD. "From alpha to beta: identification of amino acids requied for the N-acetyllactosamine specific lectin-like activity of bundlin" Mol Microbiol. 2009, 72, 859-68.

Sun N, Sun J, Kitova EN, Klassen JS. "Identifying Nonspecific Ligand Binding in Electrospray Ionization Mass Spectrometry Using the Reporter molecule Method" J Am Soc Mass Spectrom. 2009, 20, 1242-1250.

Shoemaker GK, Soya N, Palcic MM, Klassen JS. "Temperature-dependent cooperativity in donor-accepator substrate bingding to the human blood group glycosltransferases" Glycobiology 2008, 18, 587-92.

Dingle T, Wee S, Mulvey GL, Greco A, Kitova EN, Sun J, Lin S, Klassen JS, Palcic MM, Ng KK, Armstrong GD "Functional properties of the carboxy-terminal host cell-binding domais of the two toxins, TcdA and TcdB, expressed by Clostridium difficile" Glycobiology 2008, 18,698-706.

P.I. Kitov, T. Lipinski, E. Paszkiewicz, D. Solomon, J.M. Sadowska, G.A. Grant, G.L. Mulvey, E.N. Kitova, J.S. Klassen, G.D. Armstrong and D.R. Bundle "An entropically efficient supramolecular inhibition strategy for Shiga toxins" Angew. Chemie Int. Ed. 2008, 47, 672-676.

E.N. Kitova, M. Seo, P.-N. Roy and J.S. Klassen "Elucidating the Intermolecular Interactions within a Desolvated Protein-Ligand Complex. An Experimental and Computational Study" J. Am. Chem. Soc. 2008, 130, 1214-1226.

R.M. Hyland, J.Sun, T.P. Griener, G.L. Mulvey, J.S. Klassen, M.S. Donnenberg, G.D. Armstrong "The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine specific lectin" Cell. Microbiol. 2008, 10, 177-187.

A. Faridmoayer, M.A. Fentabil, D.C. Mills, J.S. Klassen and M.F. Feldman "Functional Characterization of Bacterial Oligosaccharyltransferases Involved in O-linked Protein Glycosylation" J. Bacteriol. 2007, 189, 8088-8098.

J. Sun, E.N. Kitova, N. Sun and J.S. Klassen "Method for Identifying Nonspecific Protein-Protein Interactions in Nanoelectrospray Ionization Mass Spectrometry" Anal. Chem. 2007, 79, 8301-8311.

E.N. Kitova, P.I. Kitov, E. Paszkiewicz, J. Kim, G.L. Mulvey, G.D. Armstrong, D.R. Bundle and J.S. Klassen "Affinities of Shiga Toxins 1 and 2 for Univalent and Oligovalent Pk Trisaccharide Analogs Measured by Electrospray Ionization Mass Spectrometry" Glycobiology 2007, 17, 1127-1137.

C. Rademacher, G.K. Shoemaker, H.-S. Kim, R.B. Zheng, H. Taha, C. Liu, R.C. Nacario, D.C. Schriemer, J.S. Klassen, T. Peters and T.L. Lowary "Ligand specificity of CS-35, a monoclonal antibody that recognizes mycobacterial lipoarabinomannan. A model system for oligofuranoside-protein recognition" J. Am. Chem. Soc. 2007, 129, 10489-10502.

G.K. Shoemaker, E.N. Kitova, M.M. Palcic and J.S. Klassen "Equivalency of Binding Sites in Protein-Ligand Complexes Revealed by Time-Resolved Tandem Mass Spectrometry " J. Am. Chem. Soc. 2007, 129, 8674-8675.

I. Sinelnikov, E.N. Kitova, G.D. Armstrong and J.S. Klassen "Effects of Single Amino Acid Substitution on the Dissociation of Multiply Charged Multiprotein Complexes in the Gas Phase" J. Am. Soc. Mass Spectrom. 2007, 18, 688-692.

I. Sinelnikov, E.N. Kitova and J.S. Klassen "Influence of Coulombic Repulsion on Dissociation Pathways and Energetics of Multiprotein Complexes in the Gas Phase" J. Am. Soc. Mass Spectrom. 2007, 18, 617-631.

J. Sun, E.N. Kitova and J.S. Klassen "Method for Stabilizing Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry" Anal. Chem. 2007, 79, 417-425.

G.K. Shoemaker, E.N. Kitova, and J.S. Klassen "Protein - Carbohydrate Affinities: An Example of a Direct Method to Determine Equilibrium Constants" The Encyclopedia of Mass Spectrometry 2006, Vol. 6 (Editors: M. L. Gross and R. M. Caprioli), Elsevier, Amsterdam , 810-822.