The Klassen Group

Liu, Lan; Michelsen, Klaus; Kitova, Elena; Schnier, Paul; Klassen, John "Energetics of Lipid Binding in a Hydrophobic Protein Cavity" J. Am. Chem. Soc. 2012, Accepted

 
El-haweit, Amr; Shoemaker, Glen; Daneshfar, Rambod; Kitova, Elena; Klassen, John "Applications of a Catch and Release Electrospray Ionization Mass Spectrometry Assay for Carbohydrate Library Screening" Anal. Chem 2011,Accepted

 

Elena N. Kitova, Amr El-Hawiet, Paul D. Schnier and John S. Klassen "Reliable Determinations of Protein-Ligand Interactions by Direct ESI-MS Measurements. Are We There Yet?" J. Am. Soc. Mass Spectrom 2011, Accepted

 

Mahendra S. Sandbhor, Naoto Soya, Amgad Albohy, R. Blake Zheng, Jonathan Cartmell, David R. Bundle, John S. Klassen, and Christopher W. Cairo " Substrate Recognition of the Membrane-Associated Sialidase NEU3 Requires a Hydrophobic Aglycone " Biochemistry 2011, 50, 6753-6762

 

Ping Zhang, Nahid Razi, Luiz Eugenio, Messele Fentabil, Elena N. Kitova, John S. Klassen, David R. Bundle, Kenneth K.-S. Ng and Chang-Chun Ling "Ligands for C. difficile toxins:Unexpected structure of a pentasaccharide produced by enzymatic fucosylation and corrected annotation in a popular screening library" Chem. Comm. 2011, Accepted

 

Isabelle Hug, Blake Zheng, Bela Reiz, Randy Whittal, Messele A. Fentabil, John S. Klassen, and Mario F. Feldman "Exploiting bacterial glycosylation machineries for the synthesis of a
Lewis antigen-containing glycoprotein " J. Biol. Chem. 2011, Accepted

 

Messele A. Fentabil, Rambod Daneshfar, Elena N. Kitova and John S. Klassen " Blackbody Infrared Radiative Dissociation of Protonated Oligosaccharides " J. Am. Soc. Mass Spectrom 2011, Accepted

Kitova EN, Soya N, Klassen JS. "Identifying Specific Small Molecule Interactions Using
Electrospray Ionization Mass Spectrometry" Anal. Chem. 2011, 83, 5160–5167

 

Amr El-Hawiet, Elena N. Kitova, Pavel Kitov, Luiz Eugenio, Kenneth K.S. Ng, George L. Mulvey, Tanis C. Dingle, Adam Szpacenko, Glen D. Armstrong and John S. Klassen "Binding of Clostridium difficile Toxins to Human Milk Oligosaccharides" Glycobiology, 2011, 21,1217-1227 

 

Liu L, Michelsen K, Kitova EN, Schnier PD, Klassen JS. "Evidence that Water Can Reduce the Kinetic Stability of Protein-Hydrophobic Ligand Interaction" J. Am. Chem. Soc. , 2010, 132, 17658-17660

 

Soya N, Fang Y, Palcic MM, Klassen JS. "Trapping and Characterization of Covalent Intermediates of Mutant Retaining Glycosyltransferases" Glycobiology , 2011, 21, 547-552

 

Liu L, Kitova EN, Klassen JS. "Quantifying Protein-Fatty Acid Interactions using Electrospray Ionization Mass Spectrometry " J Am Soc Mass Spectrom, 2011, 22, 310-318

 

Hakansson K and Klassen JS. "Ion Activation Methods for Tandem Mass Spectrometry" Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities, and Biological Applications, Second Edition 2010, Chapter 16 (Editor: Richard B. Cole), WILEY, 571-630.

 

El-Hawiet A, Kitova EN, Liu L, Klassen JS. "Quantifying Labile Protein-Ligand Interactions using Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom 2010, 21, 1893-1899

 

Deng L, Sun N, Kitova EN, Klassen JS. "Direct Quantification of Protein-Metal Ion Affinities by Electrospray Ionization Mass Spectrometry" Anal. Chem. 2010, 82, 2170-2174

 

Sun N, Soya N, Kitova EN, Klassen JS. "Nonspecific Interactions between Proteins and Charged Biomolecules in Electrospray Ionization Mass Spectrometry" J Am Soc Mass Spectrom 2010, 21, 472-481

 

Liu L, Bagal D, Kitova EN, Schnier PD, Klassen JS. "Hydrophobic Protein - Ligand Interactions Preserved in the Gas Phase" J. Am. Chem. Soc 2009, 131, 15980-15981.

 

Soya N, Shoemaker GK, Palcic MM, Klassen JS. "Comparative Study of Substrate and Product Binding to the Human ABO(H) Blood Group Glycosyltransferases" Glycobiology 2009, 19, 1224-1234.

 

Bagal D, Kitova EN, Liu L, El-Hawiet A, Schnier PD, Klassen JS. "Gas Phase Stabilization of Noncovalent Protein Complexes Formed by Electrospray Ionization" Anal. Chem. 2009, 81, 7801-7806.

 

Kitova EN, Mulvey GL, Dingle T, Sinelnikov I, Wee S, Griener TP, Armstrong GD, Klassen JS. "Assembly and stability of the shiga toxins investigated by electrospray ionization mass spectrometry" Biochemistry 2009, 48, 5365-74.

 

Humphries RM, Donnenbberg MS, Strecher J, Kitova EN, Klassen JS, Cui L, Griener TP, Mulvey GL, Armstrong GD. "From alpha to beta: identification of amino acids requied for the N-acetyllactosamine specific lectin-like activity of bundlin " Mol Microbiol. 2009, 72, 859-68.

 

Sun N, Sun J, Kitova EN, Klassen JS. "Identifying Nonspecific Ligand Binding in Electrospray Ionization Mass Spectrometry Using the Reporter molecule Method " J Am Soc Mass Spectrom. 2009, 20, 1242-1250.

 

Shoemaker GK, Soya N, Palcic MM, Klassen JS. "Temperature-dependent cooperativity in donor-accepator substrate bingding to the human blood group glycosltransferases " Glycobiology 2008, 18, 587-92.

 

Dingle T, Wee S, Mulvey GL, Greco A, Kitova EN, Sun J, Lin S, Klassen JS, Palcic MM, Ng KK, Armstrong GD "Functional properties of the carboxy-terminal host cell-binding domais of the two toxins, TcdA and TcdB, expressed by Clostridium difficile" Glycobiology 2008, 18,698-706.

 

P.I. Kitov, T. Lipinski, E. Paszkiewicz, D. Solomon, J.M. Sadowska, G.A. Grant, G.L. Mulvey, E.N. Kitova, J.S. Klassen, G.D. Armstrong and D.R. Bundle " An entropically efficient supramolecular inhibition strategy for Shiga toxins" Angew. Chemie Int. Ed. 2008, 47, 672-676.

 

E.N. Kitova, M. Seo, P.-N. Roy and J.S. Klassen "Elucidating the Intermolecular Interactions within a Desolvated Protein-Ligand Complex. An Experimental and Computational Study " J. Am. Chem. Soc. 2008, 130, 1214-1226.

 

R.M. Hyland, J.Sun, T.P. Griener, G.L. Mulvey, J.S. Klassen, M.S. Donnenberg, G.D. Armstrong "The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine specific lectin." Cell. Microbiol. 2008, 10, 177-187.

 

A. Faridmoayer, M.A. Fentabil, D.C. Mills, J.S. Klassen and M.F. Feldman "Functional Characterization of Bacterial Oligosaccharyltransferases Involved in O-linked Protein Glycosylation" J. Bacteriol. 2007, 189, 8088-8098.

 

J. Sun, E.N. Kitova, N. Sun and J.S. Klassen "Method for Identifying Nonspecific Protein-Protein Interactions in Nanoelectrospray Ionization Mass Spectrometry." Anal. Chem. 2007, 79, 8301-8311.

 

E.N. Kitova, P.I. Kitov, E. Paszkiewicz, J. Kim, G.L. Mulvey, G.D. Armstrong, D.R. Bundle and J.S. Klassen "Affinities of Shiga Toxins 1 and 2 for Univalent and Oligovalent Pk Trisaccharide Analogs Measured by Electrospray Ionization Mass Spectrometry." Glycobiology 2007, 17, 1127-1137.

 

C. Rademacher, G.K. Shoemaker, H.-S. Kim, R.B. Zheng, H. Taha, C. Liu, R.C. Nacario, D.C. Schriemer, J.S. Klassen, T. Peters and T.L. Lowary "Ligand specificity of CS-35, a monoclonal antibody that recognizes mycobacterial lipoarabinomannan. A model system for oligofuranoside-protein recognition." J. Am. Chem. Soc. 2007, 129, 10489-10502.

 

G.K. Shoemaker, E.N. Kitova, M.M. Palcic and J.S. Klassen "Equivalency of Binding Sites in Protein-Ligand Complexes Revealed by Time-Resolved Tandem Mass Spectrometry " J. Am. Chem. Soc. 2007, 129, 8674-8675.

 

I. Sinelnikov, E.N. Kitova, G.D. Armstrong and J.S. Klassen "Effects of Single Amino Acid Substitution on the Dissociation of Multiply Charged Multiprotein Complexes in the Gas Phase" J. Am. Soc. Mass Spectrom. 2007, 18, 688-692.

 

I. Sinelnikov, E.N. Kitova and J.S. Klassen "Influence of Coulombic Repulsion on Dissociation Pathways and Energetics of Multiprotein Complexes in the Gas Phase" J. Am. Soc. Mass Spectrom. 2007, 18, 617-631.

 

J. Sun, E.N. Kitova and J.S. Klassen " Method for Stabilizing Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry" Anal. Chem. 2007, 79, 417-425.

 

G.K. Shoemaker, E.N. Kitova, and J.S. Klassen "Protein - Carbohydrate Affinities: An Example of a Direct Method to Determine Equilibrium Constants" The Encyclopedia of Mass Spectrometry 2006, Vol. 6 (Editors: M. L. Gross and R. M. Caprioli), Elsevier, Amsterdam , 810-822.

 
J. Sun, E.N. Kitova, W. Wang and J.S. Klassen " Method for Distinguishing Specific and Nonspecific Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry" Anal. Chem. 2006, 78, 3010-3018.

 
R. Daneshfar and J.S. Klassen "Thermal Decomposition of Multiply Charged T-Rich Oligonucleotide Anions in the Gas Phase. Influence of Internal Solvation on the Arrhenius Activation Parameters for Neutral Base Loss" J. Am. Soc. Mass Spectrom. 2006, 17, 1229-1238.

 
E.N. Kitova, R. Daneshfar, P. Marcato, G. Mulvey, G.D. Armstrong and J.S. Klassen " Stability of the Homopentameric B Subunits of Shiga Toxins 1 and 2 in Solution and the Gas Phase as Revealed by NanoES-FT-ICR/MS" J. Am. Soc. Mass Spectrom. 2005, 16, 1957-1968.

 
W. Wang, E.N. Kitova, J. Sun and J.S. Klassen " Blackbody Infrared Radiative Dissociation Of Nonspecific Protein-Carbohydrate Complexes Produced by Nanoelectrospray Ionization. The Nature of the Noncovalent Interactions" J. Am. Soc. Mass Spectrom. 2005, 16, 1583-1594.

 
W. Wang, E.N. Kitova and J.S. Klassen " Nonspecific Protein-Carbohydrate Complexes Produced by Nanoelectrospray Ionization. Factors Influencing their Formation and Stability" Anal. Chem. 2005, 77, 3060-3071.

 
E.N. Kitova, D.R. Bundle and J.S. Klassen "Partitioning of Solvent Effects and Intrinsic Interactions in Biological Recognition" Angew. Chemie Int. Ed. 2004, 43, 4183-4186.

 
R. Daneshfar, E.N. Kitova and J.S. Klassen " Determination of Protein-Ligand Association Thermochemistry Using Variable-Temperature Nanoelectrospray Mass Spectrometry" J. Am. Chem. Soc. 2004, 126, 4786-4787.

 
R. Daneshfar and J.S. Klassen " Arrhenius Activation Parameters for the Loss of Neutral Nucleobases from Deprotonated Oligonucleotide Anions in the Gas Phase" J. Am. Soc. Mass Spectrom. 2004, 15, 55-64.

 
W. Wang, E.N. Kitova and J.S. Klassen " Bioactive Recognition Sites May Not Be Energetically Preferred in Protein-Carbohydrate Complexes in the Gas Phase" J. Am. Chem. Soc. 2003, 125, 13630-13631.

 
W. Wang, E.N. Kitova and J.S. Klassen " Influence of Solution and Gas Phase Processes on Protein-Carbohydrate Binding Affinities Determined by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry" Anal. Chem. 2003, 75, 4945-4955.

 
W. Wang, E.N. Kitova and J.S. Klassen " Determination of Protein-Oligosaccharide Binding by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry" Methods in Enzymology 2003, 362, Editors: Y.C. Lee and R.T. Lee, Elsevier, Amsterdam, 376-397.

 
E.N. Kitova, W. Wang, D.R. Bundle and J.S. Klassen "Retention of Bioactive Ligand Conformation in a Gaseous Protein-Trisaccharide Complex" J. Am. Chem. Soc. 2002, 124, 13980-13981.

 
N. Felitsyn, E.N. Kitova and J.S. Klassen " Thermal Decomposition of the Protein Homodimer Ecotin in the Gas Phase " J. Am. Soc. Mass Spectrom. 2002, 13, 1432-1442.

 
E.N. Kitova, D.R. Bundle and J.S. Klassen " Evidence for the Preservation of Specific Intermolecular Interactions in Gaseous Protein-Oligosaccharide Complexes" J. Am. Chem. Soc. 2002, 124, 9340-9341.

 
E.N. Kitova, D.R. Bundle and J.S. Klassen " Thermal Dissociation of Protein-Oligosaccharide Complexes in the Gas Phase. Mapping the Intrinsic Intermolecular Interactions" J. Am. Chem. Soc.  2002, 124, 5902-5913.

 
N. Felitsyn, E.N. Kitova and J.S. Klassen " Thermal Decomposition of a Gaseous Multiprotein Complex Studied by Blackbody Infrared Radiative Dissociation. Investigating the Origin of the Asymmetric Dissociation Behaviour " Anal. Chem. 2001, 73, 4647-4661.

 
E.N. Kitova, P. Kitov, D.R. Bundle and J.S. Klassen " The Observation of Multivalent Complexes of the Shiga-Like Toxin with Globotriaoside and the Determination of their Stoichiometry by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry" Glycobiology  2001, 11, 605-611.

 
  University of Alberta Chemistry at U of A